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School / Prep
ENSTBB
Internal code
BIO7-MTPMG
Description
Master the fundamentals of molecular biology for recombinant protein expression, from cloning strategy to expression assay. Cloning a DNA sequence into a plasmid. Development of a strategy for identifying positive clones.
Teaching hours
- PRACTICAL WORKPractical work72h
Mandatory prerequisites
S7 course: USP Genetic Engineering Methodology III
Further information
Practical work in molecular biology. Cloning and expression of a recombinant protein: choice of host, vector, sequence, bacterial transformation, expression test.
Assessment of knowledge
Initial assessment / Main session - Tests
| Type of assessment | Type of test | Duration (in minutes) | Number of tests | Test coefficient | Eliminatory mark in the test | Remarks |
|---|---|---|---|---|---|---|
| Integral Continuous Control | Continuous control | 1 |
Second chance / Catch-up session - Tests
| Type of assessment | Type of test | Duration (in minutes) | Number of tests | Test coefficient | Eliminatory mark in the test | Remarks |
|---|---|---|---|---|---|---|
| Final test | Written | 1 | Authorized equipment: calculator |