Separation

Explain and describe the different techniques for fractionation, detection and quantification of biological molecules.
Select a technique to answer a given problem, and justify this choice.

S5 biochemistry courses and general chemistry concepts

Fractionation methods, detection and quantification techniques
- Fractionation methods (5 self-paced lessons and 2 face-to-face lessons)

chromatography (principles, partition chromatography, adsorption chromatography, reversed phase, ion exchange, exclusion, affinity, HPLC, CPG, CCM, presentation of equipment, molecule derivation methods to facilitate analysis)
electrophoresis

- Detection and quantification techniques (1 independent class slot)

enzymatic methods (enzymatic assays, presentation of available kits)
immunological methods (antigen-antibody reaction, RIA, ELISA

The course content for Part 1 will be posted on the Moodle platform. A forum will be set up for questions, which will be answered regularly by the teacher. Two evaluations (in the form of questions and answers) will be organized at mid-course and at the end of the face-to-face sessions. The various techniques presented will be illustrated through application exercises carried out in TD (4 ½ group sessions and 1 full class session).

Physical and Analytical Chemistry

Techniques de l'ingénieur Biosciences et Techniques - " Principes des méthodes d'analyse biochimique " Handbooks " Protein Purification " website http://www.gelifesciences.com Website http://www.sge.com. Website http://www.chem.agilent.com Methods in Enzymology, guide to protein purification site http://www.elisa-tek.com
" enzymatic determination of carbohydrates ", Colonna et Hoebler, édition Wiley site http://www.libios.fr
Mass Spectrometry. Principles and Applications, E. De Hoffmann, J. Charrette, V. Stroobant, Masson Editeur, Paris, 1996. ISBN 2-225-85252-9