School / Prep
ENSMAC
Internal code
PB5TPBIO
Description
Know and correctly use the room and equipment provided for practical biochemistry work
Apply methods for analyzing water and proteins in food.
Teaching hours
- PRACTICAL WORKPractical work16h
Mandatory prerequisites
EU Structural Biochemistry courses
Syllabus
Practical exercise 0: Good laboratory practice (No grade) (L. Daury-Joucla, C. Bosch-Bouju)
This practical exercise introduces the equipment available in the biochemistry laboratory and its use.
Good laboratory practice.
Instructions for formatting results.
Practical work 1: Learning colorimetric assays (C. Bosch-Bouju, L. Daury-Joucla)
The aim of this practical work session is to give students a sense of responsibility for the work to be carried out.
Starting with an element to be assayed, students construct and carry out an assay protocol, prepare a standard range and choose the appropriate dilutions.
CR to be handed in at the end of the session: 90% of the mark
QCM de TP : 10 % de la note
This is individual work.
Practical exercise 2: Water (K. Touyarot)
Determination of the water content of simple foods (jam, yoghurt, flour) using various techniques (steaming, halogen lamp). Determination of aw.
Final CR: 100% of mark
TP 3: Proteins (M-L. Jobin)
Analysis of proteins in simple solutions (Bradford assay) or in complex foods (Kjeldahl method).
Final CR: 100% of mark
Further information
Biochemistry and Food Technology
Assessment of knowledge
Initial assessment / Main session - Tests
Type of assessment | Type of test | Duration (in minutes) | Number of tests | Test coefficient | Eliminatory mark in the test | Remarks |
---|---|---|---|---|---|---|
Continuous control | Minutes | 3 | 0.33 |