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TP Biochemistry

  • School / Prep

    ENSMAC

Internal code

PB5TPBIO

Description

Know and correctly use the room and equipment provided for practical biochemistry work
Apply methods for analyzing water and proteins in food.

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Teaching hours

  • PRACTICAL WORKPractical work16h

Mandatory prerequisites

EU Structural Biochemistry courses

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Syllabus

Practical exercise 0: Good laboratory practice (No grade) (L. Daury-Joucla, C. Bosch-Bouju)
This practical exercise introduces the equipment available in the biochemistry laboratory and its use.
Good laboratory practice.
Instructions for formatting results.

Practical work 1: Learning colorimetric assays (C. Bosch-Bouju, L. Daury-Joucla)
The aim of this practical work session is to give students a sense of responsibility for the work to be carried out.
Starting with an element to be assayed, students construct and carry out an assay protocol, prepare a standard range and choose the appropriate dilutions.
CR to be handed in at the end of the session: 90% of the mark
QCM de TP : 10 % de la note
This is individual work.

Practical exercise 2: Water (K. Touyarot)
Determination of the water content of simple foods (jam, yoghurt, flour) using various techniques (steaming, halogen lamp). Determination of aw.
Final CR: 100% of mark

TP 3: Proteins (M-L. Jobin)
Analysis of proteins in simple solutions (Bradford assay) or in complex foods (Kjeldahl method).
Final CR: 100% of mark

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Further information

Biochemistry and Food Technology

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Assessment of knowledge

Initial assessment / Main session - Tests

Type of assessmentType of testDuration (in minutes)Number of testsTest coefficientEliminatory mark in the testRemarks
Continuous controlMinutes30.33