Chemical analysis

Chromatographic techniques
Introduce the two main chromatographic techniques (gas and liquid chromatography) and their equipment, and determine the conditions for analyzing a simple mixture.

Spectroscopic techniques
Understand the principle of an NMR experiment, using the example of proton NMR.
Be able to interpret a proton and carbon-13 NMR spectrum.
Use NMR spectroscopy to identify and analyze organic compounds.
Explain the principle of the mass spectrometry technique (equipment, ionization methods).
Interpret a mass spectrum to identify a crude formula.
Determine a chemical structure by combining information extracted from various analysis spectra (IR, NMR, MS).

Basic chemistry (product classes) - some knowledge of physical chemistry (spectroscopy in particular),
Organic chemistry: knowledge of organic functions, chemical structures, polarity.

Chromatography course: Liquid and gas chromatography (12h, Anchi 1)
Introduction - presentation of the different chromatographies - potential of the techniques (9h20)
1. Gas chromatography
1.1 Introduction
1.2 Apparatus
1.3 Detectors
1.4 Fundamental quantities
1.5 Stationary phases
1.6 Separation set-up
1.7 Applications
2. Liquid chromatography (same plan as gas chromatography
3. Quantitative analysis methods: internal standardization, internal standard, external standard, dosed addition method
Application exercises on different chromatographic techniques P. Loubet and A Thienpont (2h40)

Sandra Pinet's course: Nuclear Magnetic Resonance Spectroscopy (8h, Anchi 2)
NMR principles (2h40 lecture)
1. Simplified description of the phenomenon
2. Quantum description of the phenomenon
2.1 General case
2.2 Case of the hydrogen atom
3. Obtaining a proton NMR spectrum and characteristic gradeurs
3.1 Resonance frequency
3.2 Chemical shift
3.3 Integral
4 Factors influencing chemical shift
4.1 Electronegativity
4.2 Hybridization
4.3 Anisotropy
5. Spin-spin coupling
6. Chemical exchange
7. Carbon 13 NMR

TD ^{1H 13C}NMR (5h20, I. Gosse or Y. Nicolas and S. Pinet)

Lecture by Yohann Nicolas: Mass Spectrometry (5h20, Anchi 3)
Principle of mass spectroscopy (2h40 lecture)
Ionization equipment and techniques
Molecular peak and isotopic clusters
Exercises: MCQs on the lecture and independent spectra studies (moodle)

TD de spectroscopie de masse (2h40, I. Gosse or S. Pinet, and Y. Nicolas)

TD d'Analyses Multi-techniques, I. Gosse or S. Pinet and Y. Nicolas. Pinet and Y. Nicolas (5h20, Anchi 4)

Physical and Analytical Chemistry

Chromatographies en phase liquide et supercritique by R. Rosset, M. Caude, A. Jardy
Chromatographie en phase gazeuse by Tranchant
Identification spectrométrique de composés organiques, by SILVERSTEIN, BASLER and MORILL, éditions De Boeck