TP Chemical analysis

Master the use of a chromatography device (gas or liquid),
Consider the parameters to be taken into account to set up a chromatographic analysis
Master the immediate analysis and separation of a simple mixture,
Prepare a sample,
Identify and measure the constituents of a mixture.

Chromatographic chemical analysis course: module 1A S6

Each student will work in pairs with one of his or her colleagues for 4 sessions, during which he or she will be able to apply the various chromatographic techniques seen in class: gas chromatography, thin-layer chromatography, open-column liquid chromatography with TLC follow-up of collected samples, high-performance liquid chromatography.
Students will learn to:

Operate the various types of equipment,
Prepare samples,
Use different chromatographic media,
Set up the various parameters that govern a separation,
See the influence of different mobile phases,
Identify and determine the constituents of a mixture.
carry out a quantitative analysis

CPG: (4h) separation and identification of the constituents of a mixture

Principle of dosing a constituent

HPLC: (4h) separation and identification of the constituents of a mixture - Influence of the wavelength - use of a diode-rod UV - Influence of the nature of the mobile phase

Principle of dosing a constituent.
Open column: separation of a mixture obtained after an organic synthesis on a silica column. Follow-up of the separation. Check the purity and composition of each compound obtained. Separation efficiency.

Physical and Analytical Chemistry

Chromatographies en phase liquide et supercritique by R. Rosset, M. Caude, A. Jardy
Chromatographie en phase gazeuse by Tranchant
Identification spectrométrique de composés organiques, by SILVERSTEIN, BASLER and MORILL, éditions De Boeck