Chromatography techniques

Know the two main chromatography techniques (gas phase and liquid phase)
Be able to determine the analytical conditions for a simple mixture
Be able to quantify each of the elements present in the analyzed mixtures

Basic chemistry (product classes) - some knowledge of physical chemistry (particularly spectroscopy)
Organic chemistry: knowledge of organic functions, chemical structures, polarity

Introduction
Part II: Fundamental quantities in chromatography

Retention time and retention factor
Selectivity
Van Deemter's and Golay's equations
Theoretical plate numbers
Solving
Application exercises

Part II: Gas chromatography

Introduction to the apparatus : characteristics of carrier gases, injector, detectors
Presentation of stationary phases and their characteristics
Kovats and Mac Reynolds indices
Setting up a separation of a mixture by gas chromatography
Applications
Application exercises

Part III: Liquid chromatography

Presentation of the apparatus: characteristics of solvents, injector, detectors
Presentation of stationary phases and their characteristics
Characteristics of mobile phases and polarity
Separation of a mixture by liquid chromatography
Applications
Application exercises

Part IV: Quantification

Internal standardization
External calibration
Internal calibration
Application exercises

Part V: Equipment handling sessions
1 . Separation of a mixture in gas chromatography

Isothermal mode
Setting up a temperature gradient for separation
Influence of the stationary phase
Qualitative analysis of compounds in a mixture

2. Implementing a separation of a mixture in Liquid Chromatography

Isocratic mode
Implementing an elution gradient
Influence of stationary phase, mobile phase and detection conditions

Physical and Analytical Chemistry

Liquid and supercritical phase chromatography by R. Rosset, M. Caude, A. Jardy
Gas phase chromatography by Tranchant