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TP Bioconversion and Purification (BP)

  • School / Prep

    ENSTBB

Internal code

BT8TPBPU

Description

Bioconversion: design a flow chart to calculate yields at each stage of a production process, establish a material balance.Purification: at the end of the course, students will be able to apply their operational skills in purification on a laboratory and pilot scale, and in characterizing a protein.

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Teaching hours

  • PRACTICAL WORKPractical work36h

Mandatory prerequisites

S8 Teaching: -Analytical sciences: Bioassay-DSP Purification strategies

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Further information

Production of an amino acid by bioconversion: enzyme immobilization, purification by frontal filtration, crystallization, quantification by enzymatic assay. Purification of a recombinant protein: from cell to protein, characterization by static and dynamic light scattering, pilot-scale purification.

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Assessment of knowledge

Initial assessment / Main session - Tests

Type of assessmentType of testDuration (in minutes)Number of testsTest coefficientEliminatory mark in the testRemarks
Integral Continuous ControlMinutes1

Second chance / Catch-up session - Tests

Type of assessmentType of testDuration (in minutes)Number of testsTest coefficientEliminatory mark in the testRemarks
Final testWritten1