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DSP Biomolecule purification and analysis techniques

  • School / Prep

    ENSTBB

Internal code

BT5TPTPB

Description


At the end of the Protein Purification course, students will be able to apply their knowledge of strategies for purifying and quantifying proteins by electrophoresis under denaturing conditions. He/she will be able to operate a purification automaton (AKTA Purifier, Explorer, Pure), pilot an automaton thanks to practical experience and on-line training (e-learning), and perform column packing.
Introduction to nucleic acid purification techniques: know how to purify and characterize a bacterial plasmid detect a nucleic acid using the PCR reaction use restriction enzymes and perform agarose gel electrophoresis.
Enzymes: set up and validate an enzyme test.

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Teaching hours

  • PRACTICAL WORKPractical work52h

Further information


Protein Purification, Electrophoretic Analysis,
Nucleic Acids
Enzymes

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Assessment of knowledge

Initial assessment / Main session - Tests

Type of assessmentType of testDuration (in minutes)Number of testsTest coefficientEliminatory mark in the testRemarks
Final inspectionMinutes1
Final inspectionWritten601

Second chance / Catch-up session - Tests

Type of assessmentType of testDuration (in minutes)Number of testsTest coefficientEliminatory mark in the testRemarks
Final testWritten1